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The next section, the data bits section, is a drop-down menu that allows you to select the number of data bits that you want to use for each character that is transmitted over this connection. Most often this will either be a 7 or an 8, depending on what the destination device is set to use. During this phase you will want to select 8 data bits. The next section of this phase allows you to set the error-checking data parity bits used for this connection. Five options are presented during this phase. To establish a console connection to your VPN Router, you will want to select none from the drop-down menu. The fourth section is another drop-down menu that allows you to select the number of stop bits that need to be used for this connection. The stop bits are a timing mechanism that determines the time period between each character being transmitted. When setting up the connection to the VPN Router, you will select 1 stop bit. The final section of the Port Settings phase is the flow control section. This is a drop-down menu that allows you to select the method by which data flow is controlled. Whenever establishing a connection to a serial device, you will always select Hardware. Because we are setting up a connection to a serial port on the VPN Router, we will want to select Hardware for the flow control option. Finally, four buttons are available to you during this phase. The first is the Restore Defaults button. If you want to change all of your selections back to the HyperTerminal port setting defaults, you can click this button at any time and it returns you to factory default settings.

97. E. Reissner: The effect of transverse shear deformation on the bending of elastic plates, J. Appl. Mech. 12,69-76, 1945. 98. H. Royden: ReulAncily.si.s, 3rd edition, Prentice-Hall, Englewood Cliffs, NJ, 1988 99. W. Rudin: Recil arid Cor77ple Anfi/ysis,McGraw-Hill, New York, 1986. 100. W. Rudin: Arnctional Analy.si.s, McGraw-Hill, New York, 1976.

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The field name (in this case, the parent name) becomes selected. Type new text and press the Enter key. All field names are changed to the new parent name followed by the same child names as when you copied the fields. Copy the fields. With the fields still selected, choose Edit Copy. The fields with the new names are copied to the clipboard. Paste the fields into your form. Click the Close box on the blank page file without saving the document and your original form should appear in the Document pane. Select Edit Paste and the fields are pasted into your form. Position the fields. When the fields are pasted, they remain selected. To keep them selected and easily manageable use the arrow keys on your keyboard to move the fields. Press the Shift key and strike the up-, down-, left-, and right-arrow keys to move the fields together 10 pixels at a time. Release the Shift key and you can nudge the fields into position by moving them 1 pixel at a time. Save your form. After you complete editing a form, select File Save As and overwrite your form to optimize it.

4. PHAGE LYSINS AND FUNCTIONAL SCREENING To avoid the possibility of sequence-based bias, it is ultimately necessary to identify lysin-encoding genes by the enzymatic activity of their translated proteins. This approach is the foundation of functional genomic screening, and it has become a common tool for lysin identi cation over the past decade. The experimental speci cs of lysin screening can differ slightly, and these variables are reviewed in the proceeding paragraphs. Overall, however, such methods represent variations on the same general theme: (a) phage genomic DNA is isolated and digested into fragments; (b) the fragments are ligated into an expression vector and transformed into a host organism; (c) the transformants are clonally propagated and exposed to an inducing agent to force transcription of the genomic inserts; and (d) the clones are analyzed for the acquisition of a phenotype that indicates the presence of a lysinencoding gene. For the rst three steps, the experimental considerations are fairly general in nature and unrelated to the activity of the targeted enzymes. The original source DNA can be derived from either lytic phage, isolated from the environment or purchased from commercial sources, or lysogenic prophage, induced from host bacteria with an appropriate stressing agent. For the fragmentation step, most lysin screens have utilized a standard shotgun approach to create a

The Alignment options allow you to set the axis on which the cross section will take place. Select X-Axis and you are left with either the right or left half of your object. Selecting Y-Axis leaves you with the front or back. Select the Z-Axis and you are left with the top or bottom of your object. The house in Figure 1.12 was cut along the Z-axis.

1. Open the practice file titled layers_fun.psd from the downloadable practice files on the Web site. You should see something similar to Figure 4.10. 2. Select the Move tool (V) from the toolbar. Make sure that the green triangle layer is active.

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